Reporter

Part:BBa_J100312:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2016-12-02)


repClone Red (J100205) with scrambled TetR promoter (J100311)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2212
    Illegal AgeI site found at 2324
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Assembled by inserting J100311 (scrambled TetR promoter) in repClone Red (J100205) using Golden Gate Assembly with BsaI.

References